Hotspot

rps12-clpP1

LSC · 71571-71751 · 181 bp

Back to Dissochaeta

Pi 0.0379

MarkerSeek score 80.80

Primer available yes

Label rps12-clpP1

Feature Metadata

label_name: rps12-clpP1
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 10
pi: 0.0379
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP1_p1	TGACATTTCGGATTGGCTGT	GTACAAAGAACGGGCAAACC	45.0	50.0	57.8	57.6	348-353 bp	1.0	81.4
rps12-clpP1_p2	TTTCGGATTGGCTGTCTTGT	GTACAAAGAACGGGCAAACC	45.0	50.0	57.7	57.6	343-348 bp	1.0	80.9
rps12-clpP1_p3	TGACATTTCGGATTGGCTGT	TTATGTCAGCAACGGAAGCC	45.0	50.0	57.8	58.6	290-295 bp	1.0	80.7
rps12-clpP1_p4	TTTCGGATTGGCTGTCTTGT	TTATGTCAGCAACGGAAGCC	45.0	50.0	57.7	58.6	285-290 bp	1.0	80.2
rps12-clpP1_p5	TTCGGATTGGCTGTCTTGTG	GTACAAAGAACGGGCAAACC	50.0	50.0	58.5	57.6	342-347 bp	1.0	79.2