Hotspot

trnW-CCA-trnP-UGG

LSC · 67812-68013 · 202 bp

Back to Dissochaeta

Pi 0.0132
MarkerSeek score 42.00
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
202
variable_sites
4
pi
0.0132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGTTCTACCGAACTGAACT AGACCGCTTGAAAGAAAGGG 50.0 50.0 57.9 58.1 320 bp 1.0 79.7
trnW-CCA-trnP-UGG_p2 GCGTTCTACCGAACTGAACT CTTGGTAGCGCGTTTGTTTT 50.0 45.0 57.9 57.9 289 bp 1.0 79.7
trnW-CCA-trnP-UGG_p3 ACCTACGACATCGGGTTTTG AGACCGCTTGAAAGAAAGGG 50.0 50.0 57.9 58.1 347 bp 1.0 79.4
trnW-CCA-trnP-UGG_p4 ACCTACGACATCGGGTTTTG CTTGGTAGCGCGTTTGTTTT 50.0 45.0 57.9 57.9 316 bp 1.0 79.4
trnW-CCA-trnP-UGG_p5 GCGTTCTACCGAACTGAACT TTGGTAGCGCGTTTGTTTTG 50.0 45.0 57.9 58.2 288 bp 1.0 79.2