Hotspot

petG-trnW-CCA

LSC · 67601-67737 · 137 bp

Back to Dissochaeta

Pi 0.0681

MarkerSeek score 67.40

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 14
pi: 0.0681
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACGTGGTGATCAGTTAGACC	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.0	57.9	201 bp	1.0	76.6
petG-trnW-CCA_p2	ACGTGGTGATCAGTTAGACC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.0	58.0	237 bp	1.0	76.3
petG-trnW-CCA_p3	GACGTGGTGATCAGTTAGACC	CAAAACCCGATGTCGTAGGT	52.4	50.0	58.1	57.9	202 bp	1.0	75.9
petG-trnW-CCA_p4	GACGTGGTGATCAGTTAGACC	AGCGCTCTTAGTTCAGTTCG	52.4	50.0	58.1	58.0	238 bp	1.0	75.6
petG-trnW-CCA_p5	CGTGGTGATCAGTTAGACCT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	56.7	58.0	236 bp	1.0	74.0