Hotspot

atpH-atpI

LSC · 14197-15144 · 948 bp

Back to Dissochaeta

Pi 0.0144

MarkerSeek score 69.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 20
pi: 0.0144
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.0	57.9	1007-1024 bp	1.0	81.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.0	58.1	1019-1036 bp	1.0	81.7
atpH-atpI_p3	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	1037-1054 bp	1.0	81.4
atpH-atpI_p4	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	1049-1066 bp	1.0	81.3
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.2	57.9	1008-1025 bp	1.0	80.6