Hotspot

trnS-GCU-trnR-UCU

LSC · 8088-9930 · 1843 bp

Back to Dissochaeta

Pi 0.0114

MarkerSeek score 57.00

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1843
variable_sites: 31
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ACGGAAAGAGAGGGATTCGA	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.2	55.6	1970-1974 bp	1.0	46.1
trnS-GCU-trnR-UCU_p2	ACGGAAAGAGAGGGATTCGA	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.2	54.6	1964-1968 bp	1.0	45.9
trnS-GCU-trnR-UCU_p3	TAGCAATCCGACGCTTTAGG	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.8	55.6	1919-1923 bp	1.0	45.7
trnS-GCU-trnR-UCU_p4	ACGGAAAGAGAGGGATTCGA	ACCAAAGGTTTAGAAGACCTATGT	50.0	37.5	58.2	57.6	1975-1979 bp	1.0	45.5
trnS-GCU-trnR-UCU_p5	TAGCAATCCGACGCTTTAGG	AGAAGACCTATGTCCTATCCA	50.0	42.9	57.8	54.6	1913-1917 bp	1.0	45.4