Hotspot

rps16-trnQ-UUG

LSC · 5716-6878 · 1163 bp

Back to Dissochaeta

Pi 0.029

MarkerSeek score 68.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 50
pi: 0.029
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGAATCCACGAACGATCCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1263-1273 bp	1.0	80.4
rps16-trnQ-UUG_p2	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1228-1238 bp	1.0	76.7
rps16-trnQ-UUG_p3	AAGAATCCACGAACGATCCC	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.7	57.3	1267-1277 bp	1.0	75.7
rps16-trnQ-UUG_p4	AAGAATCCACGAACGATCCC	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.7	58.7	1293-1303 bp	1.0	75.5
rps16-trnQ-UUG_p5	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	1231-1241 bp	1.0	74.5