Hotspot

rpoB

LSC · 23716-26928 · 3213 bp

Back to Disporum

Pi 0.00145

MarkerSeek score 53.60

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 9
pi: 0.00145
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	ATCCTCGGGGTTAGGTACTG	TGATAAAATCCACGGGCGTT	55.0	45.0	57.9	57.9	238 bp	1.0	79.1
rpoB_p2	CATCCTCGGGGTTAGGTACT	TGATAAAATCCACGGGCGTT	55.0	45.0	57.9	57.9	239 bp	1.0	79.1
rpoB_p3	CCTCCAATGATCGTAGCACC	TGATAAAATCCACGGGCGTT	55.0	45.0	58.1	57.9	216 bp	1.0	78.8
rpoB_p4	ATCCTCGGGGTTAGGTACTG	ATGATAAAATCCACGGGCGT	55.0	45.0	57.9	57.7	239 bp	1.0	77.3
rpoB_p5	CATCCTCGGGGTTAGGTACT	ATGATAAAATCCACGGGCGT	55.0	45.0	57.9	57.7	240 bp	1.0	77.3