Hotspot

ndhF

SSC · 111724-113922 · 2199 bp

Back to Disporum

Pi 0.00409

MarkerSeek score 35.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2199
variable_sites: 17
pi: 0.00409
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGGTCGATTTGAAAATGCCG	TGGTCATATCGTTTGATCAACCA	50.0	39.1	58.5	58.2	2778-2796 bp	1.0	52.2
ndhF_p2	ACGGTCCTTATCGGGGAATA	TGGTCATATCGTTTGATCAACCA	50.0	39.1	57.6	58.2	2424-2431 bp	0.5	32.5
ndhF_p3	TTTGAACGGTCCTTATCGGG	TGGTCATATCGTTTGATCAACCA	50.0	39.1	57.6	58.2	2429-2436 bp	0.5	32.5
ndhF_p4	AACGGTCCTTATCGGGGAAT	TGGTCATATCGTTTGATCAACCA	50.0	39.1	58.5	58.2	2425-2432 bp	0.5	31.6
ndhF_p5	ACGGTCCTTATCGGGGAATA	AGCGGAATGATGAAAATCTCA	50.0	38.1	57.6	55.7	2531-2538 bp	0.5	31.0