Hotspot

clpP

LSC · 70488-72581 · 2094 bp

Back to Disporum

Pi 0.00374

MarkerSeek score 60.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2094
variable_sites: 14
pi: 0.00374
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCAAACCACGGAAAATGCAA	TCCATTGTTACGTTTCCACACT	40.0	40.9	56.7	58.2	2201-2213 bp	1.0	53.4
clpP_p2	TCAAACCACGGAAAATGCAA	TCCATTGTTACGTTTCCACAC	40.0	42.9	56.7	56.8	2201-2213 bp	1.0	52.9
clpP_p3	TCAAACCACGGAAAATGCAA	CCATTGTTACGTTTCCACACT	40.0	42.9	56.7	56.7	2200-2212 bp	1.0	52.9
clpP_p4	ATCAAACCACGGAAAATGCA	TCCATTGTTACGTTTCCACACT	40.0	40.9	56.5	58.2	2202-2214 bp	1.0	51.6
clpP_p5	TCAAACCACGGAAAATGCAAA	TCCATTGTTACGTTTCCACACT	38.1	40.9	57.5	58.2	2201-2213 bp	1.0	51.3