Hotspot

rps15-trnN-GUU

SSC · 125249-131495 · 6247 bp

Back to Disporum

Pi 0.00521

MarkerSeek score 56.50

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 6247
variable_sites: 59
pi: 0.00521
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	GGTCTTTTCTTGTGGACGGT	TCCTAGACGTCGCAGAGAAT	50.0	50.0	58.0	58.0	1114-1116 bp	1.0	81.0
rps15-trnN-GUU_p2	GGTCTTTTCTTGTGGACGGT	CGAGGGGGTAAGAATCCTCT	50.0	55.0	58.0	57.9	670-673 bp	1.0	80.7
rps15-trnN-GUU_p3	TACGACTCAGCTCCAGCATA	TCCTAGACGTCGCAGAGAAT	50.0	50.0	57.9	58.0	1417-1419 bp	1.0	80.6
rps15-trnN-GUU_p4	TATACGACTCAGCTCCAGCA	TCCTAGACGTCGCAGAGAAT	50.0	50.0	57.9	58.0	1419-1421 bp	1.0	80.6
rps15-trnN-GUU_p5	TACGACTCAGCTCCAGCATA	CGAGGGGGTAAGAATCCTCT	50.0	55.0	57.9	57.9	973-976 bp	1.0	80.1