Hotspot

rps16-trnQ-UUG

LSC · 5770-6980 · 1211 bp

Back to Disporum

Pi 0.00433
MarkerSeek score 55.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1211
variable_sites
9
pi
0.00433
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGGCAGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1281-1302 bp 1.0 81.5
rps16-trnQ-UUG_p2 GCTTTCTGCCACATCGTTTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1251-1272 bp 1.0 81.5
rps16-trnQ-UUG_p3 AATCCACGGCAGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1287-1308 bp 1.0 81.3
rps16-trnQ-UUG_p4 GAATCCACGGCAGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1288-1309 bp 1.0 81.3
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1274-1295 bp 1.0 80.1