Hotspot

trnK-UUU-rps16

LSC · 4090-4706 · 617 bp

Back to Disporum

Pi 0.0054

MarkerSeek score 39.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 6
pi: 0.0054
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAGTGGGGTTTTACGATCCG	55.0	50.0	58.0	57.9	901-903 bp	1.0	79.8
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CGAGCCGTATGAGGAGAAAG	55.0	55.0	58.0	57.9	1034-1039 bp	1.0	79.6
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	TCGATCCCGAAGAGAAGGAA	55.0	50.0	58.0	57.9	933-935 bp	1.0	79.5
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	GAGCCGTATGAGGAGAAAGC	55.0	55.0	58.0	58.1	1033-1038 bp	1.0	79.5
trnK-UUU-rps16_p5	TCAGGATCAGTCGTGGTCTT	AAGTGGGGTTTTACGATCCG	50.0	50.0	58.1	57.9	897-899 bp	1.0	79.4