Hotspot

ycf3

LSC · 42772-44766 · 1995 bp

Back to Disporopsis

Pi 0.00209

MarkerSeek score 58.20

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1995
variable_sites: 8
pi: 0.00209
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGTCATGGTCATGGTAATGGT	CCGAGGGGGTAATGAGAAGA	42.9	55.0	57.0	58.2	2118-2120 bp	1.0	62.5
ycf3_p2	TGTCATGGTCATGGTAATGGTC	CCGAGGGGGTAATGAGAAGA	45.5	55.0	58.1	58.2	2118-2120 bp	1.0	61.7
ycf3_p3	GTCATGGTCATGGTAATGGTCT	CCGAGGGGGTAATGAGAAGA	45.5	55.0	57.8	58.2	2117-2119 bp	1.0	61.4
ycf3_p4	TCATGGTCATGGTAATGGTCTC	CCGAGGGGGTAATGAGAAGA	45.5	55.0	57.6	58.2	2116-2118 bp	1.0	59.3
ycf3_p5	CATGGTCATGGTAATGGTCTCT	CCGAGGGGGTAATGAGAAGA	45.5	55.0	57.6	58.2	2115-2117 bp	1.0	59.2