Hotspot

trnI-GAU-rrn16S

IRa · 137362-137662 · 301 bp

Back to Disporopsis

Pi 0.00498

MarkerSeek score 56.00

Primer available yes

Label trnI-GAU-rrn16S

Feature Metadata

label_name: trnI-GAU-rrn16S
feature_type: igs
region: IRa
length_bp: 301
variable_sites: 3
pi: 0.00498
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16S_p1	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	57.8	57.7	427 bp	1.0	76.9
trnI-GAU-rrn16S_p2	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	57.8	57.7	446 bp	1.0	76.8
trnI-GAU-rrn16S_p3	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	55.0	50.0	57.8	58.3	449 bp	1.0	76.7
trnI-GAU-rrn16S_p4	CGCTGATGAGCACATTGAAC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	57.8	58.3	468 bp	1.0	76.7
trnI-GAU-rrn16S_p5	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	395 bp	1.0	76.7