Hotspot

trnR-ACG-rrn5S

IRa · 131628-131883 · 256 bp

Back to Disporopsis

Pi 0
MarkerSeek score 33.80
Primer available yes
Label trnR-ACG-rrn5S

Feature Metadata

label_name
trnR-ACG-rrn5S
feature_type
igs
region
IRa
length_bp
256
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5S_p1 ACCCCCAGAGGTAAAGGAAA GGTGACGATACTGTAGGGGA 50.0 55.0 57.9 57.9 426 bp 1.0 78.4
trnR-ACG-rrn5S_p2 ACCCCCAGAGGTAAAGGAAA AATCCATCCCGAACTTGGTG 50.0 50.0 57.9 57.9 461 bp 1.0 77.8
trnR-ACG-rrn5S_p3 ACCCCCAGAGGTAAAGGAAA ACCAATCCATCCCGAACTTG 50.0 50.0 57.9 57.9 464 bp 1.0 77.8
trnR-ACG-rrn5S_p4 ACCCCCAGAGGTAAAGGAAA CAATCCATCCCGAACTTGGT 50.0 50.0 57.9 57.9 462 bp 1.0 77.8
trnR-ACG-rrn5S_p5 ACCCCCAGAGGTAAAGGAAA CACCAATCCATCCCGAACTT 50.0 50.0 57.9 57.9 465 bp 1.0 77.8