Hotspot

rrn4.5S-rrn5S

IRb · 108941-109161 · 221 bp

Back to Disporopsis

Pi 0.0226

MarkerSeek score 54.30

Primer available yes

Label rrn4.5S-rrn5S

Feature Metadata

label_name: rrn4.5S-rrn5S
feature_type: igs
region: IRb
length_bp: 221
variable_sites: 10
pi: 0.0226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5S-rrn5S_p1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	338-339 bp	1.0	81.7
rrn4.5S-rrn5S_p2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	336-337 bp	1.0	81.7
rrn4.5S-rrn5S_p3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	334-335 bp	1.0	81.7
rrn4.5S-rrn5S_p4	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	50.0	50.0	58.0	58.0	364-365 bp	1.0	81.6
rrn4.5S-rrn5S_p5	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	50.0	50.0	58.0	58.0	362-363 bp	1.0	81.6