Hotspot

petA-psbJ

LSC · 64138-65218 · 1081 bp

Back to Disporopsis

Pi 0.00509

MarkerSeek score 53.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1081
variable_sites: 11
pi: 0.00509
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.1	58.0	1186-1190 bp	1.0	80.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	1203-1207 bp	1.0	79.4
petA-psbJ_p3	GGCATCCGTTATTTTGGCAC	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.1	58.1	1187-1191 bp	1.0	79.4
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	1204-1208 bp	1.0	78.8
petA-psbJ_p5	TGTTCTTCTTGGCATCCGTT	CCGGATTGGGTTCATCTCTG	45.0	55.0	57.7	58.0	1196-1200 bp	1.0	78.5