Pi
0.00337
MarkerSeek score
65.90
Primer available
yes
Label
trnT-UGU-trnL
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL_p1 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1014-1072 bp | 1.0 | 82.2 |
| trnT-UGU-trnL_p2 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1022-1080 bp | 1.0 | 81.6 |
| trnT-UGU-trnL_p3 | AAATGCGATGCTCTAACCCC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.3 | 58.0 | 986-1044 bp | 1.0 | 81.6 |
| trnT-UGU-trnL_p4 | AAATGCGATGCTCTAACCCC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.3 | 58.1 | 994-1052 bp | 1.0 | 81.1 |
| trnT-UGU-trnL_p5 | GGAATCGAACCGATGACCAT | ATCCAATCAAGTCCGTAGCG | 50.0 | 50.0 | 57.8 | 57.8 | 1037-1095 bp | 1.0 | 80.3 |