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trnC-GCA-petN

LSC · 28608-29489 · 882 bp

Back to Disporopsis

Pi 0.00567

MarkerSeek score 49.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 10
pi: 0.00567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATTTTGGACCGAATTTGGCG	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.6	58.0	1000-1003 bp	1.0	78.1
trnC-GCA-petN_p2	ATTTTGGACCGAATTTGGCG	AGCAATTAGTAGTGCCCAGC	45.0	50.0	57.6	58.0	1077-1080 bp	1.0	77.7
trnC-GCA-petN_p3	ATTTTGGACCGAATTTGGCG	CACCCCTTCCCCATACTACA	45.0	55.0	57.6	58.1	1048-1051 bp	1.0	77.2
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.5	58.0	960-963 bp	1.0	76.8
trnC-GCA-petN_p5	ATTTTGGACCGAATTTGGCG	CAGCCCAAGCAAGACTTACT	45.0	50.0	57.6	57.8	994-997 bp	1.0	76.6