Hotspot

atpH-atpI

LSC · 14548-15047 · 500 bp

Back to Disporopsis

Pi 0.001

MarkerSeek score 53.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 1
pi: 0.001
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	531-564 bp	1.0	83.3
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.1	57.9	619-652 bp	1.0	82.9
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	587-620 bp	1.0	82.7
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	532-565 bp	1.0	82.1
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.2	57.9	591-624 bp	1.0	82.1