Hotspot

trnQ-UUG-trnR-ACG

Genome · 85555-85618 · 64 bp

Back to Discostella

Pi 0.133
MarkerSeek score 79.70
Primer available yes
Label trnQ-UUG-trnR-ACG

Feature Metadata

label_name
trnQ-UUG-trnR-ACG
feature_type
igs
region
Genome
length_bp
64
variable_sites
11
pi
0.133
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.86
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-trnR-ACG_p1 AAAGTCCACAGCCTTACCAC GGTTCGAATCCCTTCTTGCT 50.0 50.0 57.7 57.9 113-122 bp 1.0 88.5
trnQ-UUG-trnR-ACG_p2 CAAAGTCCACAGCCTTACCA GGTTCGAATCCCTTCTTGCT 50.0 50.0 57.7 57.9 114-123 bp 1.0 88.4
trnQ-UUG-trnR-ACG_p3 GAGTCAAAGTCCACAGCCTT GGTTCGAATCCCTTCTTGCT 50.0 50.0 57.7 57.9 118-127 bp 1.0 88.3
trnQ-UUG-trnR-ACG_p4 GAGTCAAAGTCCACAGCCTT GGGGTTCGAATCCCTTCTTG 50.0 55.0 57.7 58.2 120-129 bp 1.0 87.1
trnQ-UUG-trnR-ACG_p5 GTGGCAGGATTCGAACCTAC GGTTCGAATCCCTTCTTGCT 55.0 50.0 58.3 57.9 146-155 bp 1.0 86.2