Hotspot

ycf89-rns

Genome · 66371-66861 · 491 bp

Back to Discostella

Pi 0.228

MarkerSeek score 79.70

Primer available yes

Label ycf89-rns

Feature Metadata

label_name: ycf89-rns
feature_type: igs
region: Genome
length_bp: 491
variable_sites: 139
pi: 0.228
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89-rns_p1	GTTGATGCCGTAGATTGGGT	CATTGCGGAAAATTCCCCAC	50.0	50.0	58.0	58.0	1282 bp	0.333333	53.0
ycf89-rns_p2	GTTGATGCCGTAGATTGGGT	TCTCGTACGACTTGCATGTG	50.0	50.0	58.0	58.0	1000 bp	0.333333	52.8
ycf89-rns_p3	GTTGATGCCGTAGATTGGGT	TATTGCTCCGTCAGGCTTTC	50.0	50.0	58.0	58.0	1305 bp	0.333333	52.8
ycf89-rns_p4	GTTGATGCCGTAGATTGGGT	CAACCCATAGGCAGTCTTCC	50.0	55.0	58.0	58.0	1334 bp	0.333333	52.7
ycf89-rns_p5	GTTGATGCCGTAGATTGGGT	CTTGCATCCTCCGTCTTACC	50.0	55.0	58.0	58.1	1433 bp	0.333333	52.6