Hotspot

trnD-GUC-petN

Genome · 49507-49700 · 194 bp

Back to Discostella

Pi 0.172

MarkerSeek score 79.40

Primer available yes

Label trnD-GUC-petN

Feature Metadata

label_name: trnD-GUC-petN
feature_type: igs
region: Genome
length_bp: 194
variable_sites: 43
pi: 0.172
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-petN_p1	AAGTTGCGAGTTCGAGTCTC	GCCAGCCCAACCTAGACTTA	50.0	55.0	57.9	59.1	234-254 bp	1.0	82.9
trnD-GUC-petN_p2	GAAGTTGCGAGTTCGAGTCT	GCCAGCCCAACCTAGACTTA	50.0	55.0	57.9	59.1	235-255 bp	1.0	82.9
trnD-GUC-petN_p3	GAGTTCGAGTCTCGTCAGTC	GCCAGCCCAACCTAGACTTA	55.0	55.0	57.5	59.1	227-247 bp	1.0	79.2
trnD-GUC-petN_p4	TCAATTGGTTAGAGCACCGC	GCCAGCCCAACCTAGACTTA	50.0	55.0	58.6	59.1	267-287 bp	1.0	78.9
trnD-GUC-petN_p5	AAGTTGCGAGTTCGAGTCTC	ATTACGCCAGCCCAACCTA	50.0	52.6	57.9	58.7	239-259 bp	1.0	78.0