Hotspot

trnS-GCU-trnD-GUC

Genome · 49362-49432 · 71 bp

Back to Discostella

Pi 0.174

MarkerSeek score 79.80

Primer available yes

Label trnS-GCU-trnD-GUC

Feature Metadata

label_name: trnS-GCU-trnD-GUC
feature_type: igs
region: Genome
length_bp: 71
variable_sites: 18
pi: 0.174
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnD-GUC_p1	AATGGCTGAGTGGTCGAAAG	AGACTCGAACTCGCAACTTC	50.0	50.0	58.2	57.9	216-218 bp	1.0	85.1
trnS-GCU-trnD-GUC_p2	AATGGCTGAGTGGTCGAAAG	GAGACTCGAACTCGCAACTT	50.0	50.0	58.2	57.9	217-219 bp	1.0	85.1
trnS-GCU-trnD-GUC_p3	GAAATGGCTGAGTGGTCGAA	AGACTCGAACTCGCAACTTC	50.0	50.0	58.2	57.9	218-220 bp	1.0	85.0
trnS-GCU-trnD-GUC_p4	GAAATGGCTGAGTGGTCGAA	GAGACTCGAACTCGCAACTT	50.0	50.0	58.2	57.9	219-221 bp	1.0	85.0
trnS-GCU-trnD-GUC_p5	GGAGAAATGGCTGAGTGGTC	AGACTCGAACTCGCAACTTC	55.0	50.0	58.3	57.9	221-223 bp	1.0	84.4