Hotspot

ftsH-trnT-UGU

Genome · 48403-48659 · 257 bp

Back to Discostella

Pi 0.192

MarkerSeek score 74.60

Primer available yes

Label ftsH-trnT-UGU

Feature Metadata

label_name: ftsH-trnT-UGU
feature_type: igs
region: Genome
length_bp: 257
variable_sites: 70
pi: 0.192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH-trnT-UGU_p1	CTTGTACGATGGCATTCCGA	AACCCCCAACCTACGGATTA	50.0	50.0	58.1	58.0	551-556 bp	0.666667	74.8
ftsH-trnT-UGU_p2	CTTGTACGATGGCATTCCGA	CGACCTTGGGCTTATGAGTC	50.0	55.0	58.1	58.1	1065-1163 bp	0.666667	74.1
ftsH-trnT-UGU_p3	CTGGGCTAGAAGCTTGTACG	AACCCCCAACCTACGGATTA	55.0	50.0	58.1	58.0	568 bp	0.333333	52.5
ftsH-trnT-UGU_p4	CTGGGCTAGAAGCTTGTACG	CGACCTTGGGCTTATGAGTC	55.0	55.0	58.1	58.1	1077 bp	0.333333	52.3
ftsH-trnT-UGU_p5	GGAGATTGACCTGAACCACC	AACCCCCAACCTACGGATTA	55.0	50.0	57.9	58.0	797 bp	0.333333	52.2