Hotspot

rpl1-rpl12

Genome · 26120-26199 · 80 bp

Back to Discostella

Pi 0.142

MarkerSeek score 79.40

Primer available yes

Label rpl1-rpl12

Feature Metadata

label_name: rpl1-rpl12
feature_type: igs
region: Genome
length_bp: 80
variable_sites: 15
pi: 0.142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl1-rpl12_p1	TGCGAAAATTATCCCGACGT	GCCATCATAATACCCCCACC	45.0	55.0	58.0	57.8	903-909 bp	0.666667	71.7
rpl1-rpl12_p2	TGCGAAAATTATCCCGACGT	AACGACGATGCTGTACGATC	45.0	50.0	58.0	58.2	1359 bp	0.333333	51.8
rpl1-rpl12_p3	TGCGAAAATTATCCCGACGT	CGACGATGCTGTACGATCAA	45.0	50.0	58.0	58.2	1357 bp	0.333333	51.8
rpl1-rpl12_p4	TGCGAAAATTATCCCGACGT	GGCCATCATAATACCCCCAC	45.0	55.0	58.0	57.8	910 bp	0.333333	51.5
rpl1-rpl12_p5	CAATTGCGTACAACGGTGAC	AACGACGATGCTGTACGATC	50.0	50.0	58.1	58.2	1189 bp	0.333333	51.5