Hotspot

psbX-rbcS

Genome · 7545-7966 · 422 bp

Back to Discostella

Pi 0.211

MarkerSeek score 81.30

Primer available yes

Label psbX-rbcS

Feature Metadata

label_name: psbX-rbcS
feature_type: igs
region: Genome
length_bp: 422
variable_sites: 116
pi: 0.211
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbX-rbcS_p1	GCGATAAAATTTGCTAATGAAGTTGT	CCCTGAAGGTAGCCGTTATT	30.8	50.0	57.8	57.0	408-476 bp	1.0	52.0
psbX-rbcS_p2	GCGATAAAATTTGCTAATGAAGTTGT	CAGTGTTCAAGCTAACCCTGA	30.8	47.6	57.8	57.9	423-491 bp	1.0	52.0
psbX-rbcS_p3	GCGATAAAATTTGCTAATGAAGTTGT	TGTTCAAGCTAACCCTGAAGG	30.8	47.6	57.8	57.9	420-488 bp	1.0	52.0
psbX-rbcS_p4	GCGATAAAATTTGCTAATGAAGTTGT	ACAGTGTTCAAGCTAACCCTG	30.8	47.6	57.8	58.1	424-492 bp	1.0	52.0
psbX-rbcS_p5	GCGATAAAATTTGCTAATGAAGTTGT	ACAGTGTTCAAGCTAACCCT	30.8	45.0	57.8	56.7	424-492 bp	1.0	52.0