Hotspot

trnV-UAC

LSC · 57845-57883 · 39 bp

Back to Discocleidion

Pi 0.0256

MarkerSeek score 67.20

Primer available yes

Label trnV-UAC

Feature Metadata

label_name: trnV-UAC
feature_type: tRNA
region: LSC
length_bp: 39
variable_sites: 1
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC_p1	ACATGATGGACTCCCTTGAA	AGCAATACCGATCAAAGAGA	45.0	40.0	56.1	54.2	185-186 bp	1.0	40.4
trnV-UAC_p2	ACATGATGGACTCCCTTGAA	AAGCAATACCGATCAAAGAGA	45.0	38.1	56.1	55.0	186-187 bp	1.0	40.4
trnV-UAC_p3	ACATGATGGACTCCCTTGAAA	AGCAATACCGATCAAAGAGA	42.9	40.0	56.9	54.2	185-186 bp	1.0	40.4
trnV-UAC_p4	AGGCTATTGTTCTCTTGTTCCC	AGCAATACCGATCAAAGAGA	45.5	40.0	58.0	54.2	254-255 bp	1.0	40.3
trnV-UAC_p5	GGCTATTGTTCTCTTGTTCCCT	AGCAATACCGATCAAAGAGA	45.5	40.0	58.0	54.2	253-254 bp	1.0	40.3