Hotspot

ycf1-ndhF

SSC · 120131-121307 · 1177 bp

Back to Discocleidion

Pi 0.0934

MarkerSeek score 78.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 1177
variable_sites: 91
pi: 0.0934
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGGAAGAAAGGGAGGAAGA	GCGGTCGCATCTCTTCTTAT	50.0	50.0	58.0	57.9	1527-1871 bp	1.0	87.1
ycf1-ndhF_p2	CGGAAGAAAAGGAGGATCGG	GCGGTCGCATCTCTTCTTAT	55.0	50.0	58.1	57.9	1421-1765 bp	1.0	87.1
ycf1-ndhF_p3	ACGGAAGAAAGGGAGGAAGA	AATATGTAGGAAGCGGTCGC	50.0	50.0	58.0	57.8	1539-1883 bp	1.0	87.0
ycf1-ndhF_p4	GCAGAATACCGTCACCCATT	GCGGTCGCATCTCTTCTTAT	50.0	50.0	58.0	57.9	1577-1921 bp	1.0	86.9
ycf1-ndhF_p5	GCAGAATACCGTCACCCATT	AATATGTAGGAAGCGGTCGC	50.0	50.0	58.0	57.8	1589-1933 bp	1.0	86.8