Hotspot

ndhC-trnV-UAC

LSC · 56416-57220 · 805 bp

Back to Discocleidion

Pi 0.0531

MarkerSeek score 89.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 42
pi: 0.0531
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 89.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCTTTGCTAATTGGGGAT	GAACGAGCCCAATGCAAAAA	50.0	45.0	58.2	57.9	1037-1246 bp	1.0	81.1
ndhC-trnV-UAC_p2	GCCCTTTGCTAATTGGGGAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	950-1159 bp	1.0	81.0
ndhC-trnV-UAC_p3	GCCCTTTGCTAATTGGGGAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.2	57.5	949-1158 bp	1.0	78.3
ndhC-trnV-UAC_p4	GCCCTTTGCTAATTGGGGAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.2	58.7	938-1147 bp	1.0	77.2
ndhC-trnV-UAC_p5	GCCCTTTGCTAATTGGGGAT	AAGGTCTACGGTTCGAGTCC	50.0	55.0	58.2	58.8	948-1157 bp	1.0	76.0