Hotspot

psbZ-trnG-UCC

LSC · 41808-42425 · 618 bp

Back to Discocleidion

Pi 0.0177
MarkerSeek score 64.10
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
618
variable_sites
10
pi
0.0177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 CCGGTACATCATTATGGATTGGA CGTCTTCTCCTTGGCAAAGA 43.5 50.0 58.1 57.8 711-730 bp 1.0 59.0
psbZ-trnG-UCC_p2 TCCGGTACATCATTATGGATTGG CGTCTTCTCCTTGGCAAAGA 43.5 50.0 58.1 57.8 712-731 bp 1.0 59.0
psbZ-trnG-UCC_p3 CCGGTACATCATTATGGATTGG CGTCTTCTCCTTGGCAAAGA 45.5 50.0 56.7 57.8 711-730 bp 1.0 57.4
psbZ-trnG-UCC_p4 CCGGTACATCATTATGGATTGGA CGGATAGCGGGAATCGAAC 43.5 57.9 58.1 58.2 733-752 bp 1.0 50.8
psbZ-trnG-UCC_p5 TCCGGTACATCATTATGGATTGG CGGATAGCGGGAATCGAAC 43.5 57.9 58.1 58.2 734-753 bp 1.0 50.8