Hotspot

trnE-UUC-trnT-GGU

LSC · 34052-36689 · 2638 bp

Back to Discocleidion

Pi 0.0662

MarkerSeek score 85.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 2638
variable_sites: 166
pi: 0.0662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTCCGTAATACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	2779 bp	0.5	59.1
trnE-UUC-trnT-GGU_p2	TTCCGTAATACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.9	58.1	2758 bp	0.5	58.7
trnE-UUC-trnT-GGU_p3	TTCCGTAATACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.9	58.1	2755 bp	0.5	58.5
trnE-UUC-trnT-GGU_p4	TTCCGTAATACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.9	58.2	2776 bp	0.5	58.0
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	2741 bp	0.5	57.8