Hotspot

trnS-GCU-trnG-GCC

LSC · 9079-10011 · 933 bp

Back to Discocleidion

Pi 0.0292

MarkerSeek score 61.30

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 933
variable_sites: 14
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.51
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TTCATCGGAATATGGAGCCG	50.0	50.0	58.0	57.5	625-1056 bp	1.0	81.7
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TTCATCGGAATATGGAGCCG	50.0	50.0	58.0	57.5	624-1055 bp	1.0	81.6
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	GAGCCGAGGATCCCTTAACT	50.0	55.0	58.0	58.6	611-1042 bp	1.0	80.6
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	GAGCCGAGGATCCCTTAACT	50.0	55.0	58.0	58.6	610-1041 bp	1.0	80.6
trnS-GCU-trnG-GCC_p5	TTCCAAGTCATCACCGGAAC	TTCATCGGAATATGGAGCCG	50.0	50.0	57.8	57.5	698-1129 bp	1.0	80.1