Hotspot

psbA-trnK-UUU

LSC · 1745-2178 · 434 bp

Back to Discocleidion

Pi 0.0305

MarkerSeek score 70.40

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 13
pi: 0.0305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAACGACCCCATAGGCTTTC	CTATCCGACTAGTTCCGGGT	50.0	55.0	57.9	58.0	516-523 bp	1.0	82.1
psbA-trnK-UUU_p2	GAAACGACCCCATAGGCTTT	CTATCCGACTAGTTCCGGGT	50.0	55.0	57.9	58.0	517-524 bp	1.0	82.1
psbA-trnK-UUU_p3	CCAGTTACAGAAACGACCCC	CTATCCGACTAGTTCCGGGT	55.0	55.0	58.2	58.0	526-533 bp	1.0	81.4
psbA-trnK-UUU_p4	AAACGACCCCATAGGCTTTC	CGGCTTGGAGAGGGATTTTT	50.0	50.0	57.9	58.2	568-576 bp	1.0	80.8
psbA-trnK-UUU_p5	GAAACGACCCCATAGGCTTT	CGGCTTGGAGAGGGATTTTT	50.0	50.0	57.9	58.2	569-577 bp	1.0	80.8