Hotspot

rpl16

LSC · 88451-90055 · 1605 bp

Back to Dischidia

Pi 0.0166

MarkerSeek score 54.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1605
variable_sites: 59
pi: 0.0166
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCGACGATTGCTAGCTCCTA	CGCGAGGGGTTGAAGAATTA	50.0	50.0	58.0	58.0	2351-2396 bp	0.833333	77.1
rpl16_p2	ACCTCAGCAATAGTGTCCCT	CGCGAGGGGTTGAAGAATTA	50.0	50.0	58.0	58.0	2817-2863 bp	0.833333	76.7
rpl16_p3	AAAATAGCGCGATCCCCTTT	CGCGAGGGGTTGAAGAATTA	45.0	50.0	57.9	58.0	2647-2691 bp	0.833333	76.7
rpl16_p4	AAATAGCGCGATCCCCTTTT	CGCGAGGGGTTGAAGAATTA	45.0	50.0	57.9	58.0	2646-2690 bp	0.833333	76.7
rpl16_p5	AATAGCGCGATCCCCTTTTT	CGCGAGGGGTTGAAGAATTA	45.0	50.0	57.9	58.0	2645-2654 bp	0.5	60.2