Hotspot

clpP

LSC · 76975-79240 · 2266 bp

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Pi 0.0219

MarkerSeek score 58.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2266
variable_sites: 106
pi: 0.0219
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCACCAACGGAAACCATAA	CAATACGCAACGGGGACTAA	45.0	50.0	58.0	58.0	2905-2989 bp	1.0	85.9
clpP_p2	TTGCACCAACGGAAACCATA	CAATACGCAACGGGGACTAA	45.0	50.0	58.0	58.0	2906-2990 bp	1.0	85.9
clpP_p3	ACATGTTCCTCGACGTTGAG	CAATACGCAACGGGGACTAA	50.0	50.0	57.9	58.0	2705-2781 bp	1.0	85.8
clpP_p4	TGAGATTTGCACCAACGGAA	CAATACGCAACGGGGACTAA	45.0	50.0	58.0	58.0	2912-2996 bp	0.833333	78.3
clpP_p5	TTGAGATTTGCACCAACGGA	CAATACGCAACGGGGACTAA	45.0	50.0	58.0	58.0	2913-2997 bp	0.833333	78.3