Hotspot

ndhG-ndhI

IRa · 141104-141642 · 539 bp

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Pi 0.0102

MarkerSeek score 64.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: IRa
length_bp: 539
variable_sites: 12
pi: 0.0102
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATCAGACCCGATCCCAGAAA	TCAAATTGCTTTGGGTCGGT	50.0	45.0	58.1	58.3	649-680 bp	1.0	82.0
ndhG-ndhI_p2	ATCAGACCCGATCCCAGAAA	CAAATTGCTTTGGGTCGGTT	50.0	45.0	58.1	57.5	648-679 bp	1.0	80.2
ndhG-ndhI_p3	GAAAATCATGCATTGGCCCG	TCAAATTGCTTTGGGTCGGT	50.0	45.0	58.4	58.3	621-652 bp	1.0	79.7
ndhG-ndhI_p4	ATCAGACCCGATCCCAGAAA	GTCGGTTACCAACGTCAGTA	50.0	50.0	58.1	57.3	635-666 bp	1.0	78.9
ndhG-ndhI_p5	ACCCGATCCCAGAAAGACTA	TCAAATTGCTTTGGGTCGGT	50.0	45.0	57.5	58.3	644-675 bp	1.0	78.6