Hotspot

ndhI-ndhG

IRb · 126359-126897 · 539 bp

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Pi 0.0102

MarkerSeek score 64.30

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: IRb
length_bp: 539
variable_sites: 12
pi: 0.0102
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TCAAATTGCTTTGGGTCGGT	ATCAGACCCGATCCCAGAAA	45.0	50.0	58.3	58.1	649-680 bp	1.0	82.0
ndhI-ndhG_p2	CAAATTGCTTTGGGTCGGTT	ATCAGACCCGATCCCAGAAA	45.0	50.0	57.5	58.1	648-679 bp	1.0	80.2
ndhI-ndhG_p3	TCAAATTGCTTTGGGTCGGT	GAAAATCATGCATTGGCCCG	45.0	50.0	58.3	58.4	621-652 bp	1.0	79.7
ndhI-ndhG_p4	GTCGGTTACCAACGTCAGTA	ATCAGACCCGATCCCAGAAA	50.0	50.0	57.3	58.1	635-666 bp	1.0	78.9
ndhI-ndhG_p5	TCAAATTGCTTTGGGTCGGT	ACCCGATCCCAGAAAGACTA	45.0	50.0	58.3	57.5	644-675 bp	1.0	78.6