Hotspot

accD-psaI

LSC · 66084-66386 · 303 bp

Back to Dischidia

Pi 0.0312

MarkerSeek score 59.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 303
variable_sites: 20
pi: 0.0312
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGCTTACATTGCATTTGCGG	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	532-557 bp	1.0	87.7
accD-psaI_p2	ACGGCCAGTTTTGGTATGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	579-604 bp	1.0	87.3
accD-psaI_p3	TGCTTACATTGCATTTGCGG	TCTAGCGATCCTGGTCGATT	45.0	50.0	58.0	58.0	785-810 bp	1.0	86.4
accD-psaI_p4	TGCTTACATTGCATTTGCGG	CTGGTCGATTTGAAAACGGC	45.0	50.0	58.0	58.0	775-800 bp	1.0	86.2
accD-psaI_p5	ACGGCCAGTTTTGGTATGTT	TCTAGCGATCCTGGTCGATT	45.0	50.0	58.0	58.0	832-857 bp	1.0	86.0