Hotspot

ndhC-trnV-UAC

LSC · 56501-58024 · 1524 bp

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Pi 0.0232
MarkerSeek score 64.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1524
variable_sites
76
pi
0.0232
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 31.8 55.0 53.9 58.1 1560-1637 bp 1.0 47.8
ndhC-trnV-UAC_p2 AGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 33.3 55.0 56.1 58.1 1609-1686 bp 1.0 47.7
ndhC-trnV-UAC_p3 AAGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.7 58.1 1610-1687 bp 1.0 47.7
ndhC-trnV-UAC_p4 GCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 34.8 55.0 54.7 58.1 1608-1685 bp 1.0 47.7
ndhC-trnV-UAC_p5 AGCCAAAATAGGAATAAGACTTG CCGAGAAAGTCTACGGTTCG 34.8 55.0 54.7 58.1 1609-1686 bp 1.0 47.7