Hotspot

psaA-ycf3

LSC · 47676-48510 · 835 bp

Back to Dischidia

Pi 0.023

MarkerSeek score 67.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 42
pi: 0.023
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	944-974 bp	1.0	84.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	925-955 bp	1.0	84.3
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	948-978 bp	1.0	84.1
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	929-959 bp	1.0	84.1
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	AGCTGCCGAGTATTGGAAAC	50.0	50.0	58.1	58.3	1011-1041 bp	1.0	83.2