Hotspot

trnE-UUC-trnT-GGU

LSC · 36548-37533 · 986 bp

Back to Dischidia

Pi 0.0369

MarkerSeek score 71.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 986
variable_sites: 77
pi: 0.0369
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGGGAGATGTCCTGAACCTC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1029-1106 bp	1.0	88.7
trnE-UUC-trnT-GGU_p2	GGGAGATGTCCTGAACCTCT	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1028-1105 bp	1.0	88.7
trnE-UUC-trnT-GGU_p3	AGGGAGATGTCCTGAACCTC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.1	57.9	994-1071 bp	1.0	88.1
trnE-UUC-trnT-GGU_p4	GGGAGATGTCCTGAACCTCT	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.1	57.9	993-1070 bp	1.0	88.1
trnE-UUC-trnT-GGU_p5	TGAACCTCTAGACGATGGGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	1017-1094 bp	1.0	88.0