Hotspot

trnG-UCC-trnR-UCU

LSC · 10186-10352 · 167 bp

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Pi 0.0236
MarkerSeek score 61.00
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
167
variable_sites
9
pi
0.0236
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG GAAGACCCCTGTCCTATCCA 50.0 55.0 58.0 57.8 235-247 bp 1.0 85.2
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG GAAGACCCCTGTCCTATCCA 55.0 55.0 58.1 57.8 238-250 bp 1.0 84.9
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGAAGACCCCTGTCCTATCC 50.0 55.0 58.0 57.5 236-248 bp 1.0 82.8
trnG-UCC-trnR-UCU_p4 CCCTAGCCTTCCAAGCTAAC GAAGACCCCTGTCCTATCCA 55.0 55.0 57.7 57.8 239-251 bp 1.0 82.7
trnG-UCC-trnR-UCU_p5 CCTAGCCTTCCAAGCTAACG AGAAGACCCCTGTCCTATCC 55.0 55.0 58.1 57.5 239-251 bp 1.0 82.5