Hotspot

trnS-GCU-trnG-UCC

LSC · 8790-9447 · 658 bp

Back to Dischidia

Pi 0.0232

MarkerSeek score 68.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 38
pi: 0.0232
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TCAAACCGAAAACAAAGACCC	45.0	42.9	58.0	57.2	703-864 bp	1.0	73.3
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	CAAACCGAAAACAAAGACCCT	45.0	42.9	58.0	57.2	702-863 bp	1.0	73.2
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	CAAACCGAAAACAAAGACCCTT	45.0	40.9	58.0	57.9	702-863 bp	1.0	70.6
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	TCAAACCGAAAACAAAGACCC	50.0	42.9	57.6	57.2	689-850 bp	1.0	70.4
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	CAAACCGAAAACAAAGACCCT	50.0	42.9	57.6	57.2	688-849 bp	1.0	70.3