Hotspot

rps16-trnQ-UUG

LSC · 6181-7554 · 1374 bp

Back to Dischidia

Pi 0.018

MarkerSeek score 55.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1374
variable_sites: 55
pi: 0.018
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TTCTACTCGAGCTCCATCGT	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	2102-2270 bp	1.0	86.2
rps16-trnQ-UUG_p2	TTCTACTCGAGCTCCATCGT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2107-2275 bp	1.0	86.2
rps16-trnQ-UUG_p3	AACTTGCTCGAATGGGATCC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	2218-2386 bp	1.0	86.0
rps16-trnQ-UUG_p4	AACTTGCTCGAATGGGATCC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2223-2391 bp	1.0	86.0
rps16-trnQ-UUG_p5	GATTCCCGCGTGATACACTT	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.1	58.1	2288-2456 bp	1.0	85.7