Hotspot

trnK-UUU-rps16

LSC · 4245-5089 · 845 bp

Back to Dischidia

Pi 0.0169

MarkerSeek score 59.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 31
pi: 0.0169
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAGGGTGGGGGTTTTTAAGG	50.0	50.0	58.2	57.6	905-980 bp	1.0	81.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAGGGTGGGGGTTTTTAAGG	50.0	50.0	58.2	57.6	904-979 bp	1.0	81.5
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AGGGTGGGGGTTTTTAAGGA	50.0	50.0	58.2	58.5	903-978 bp	1.0	81.2
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	GGGTGGGGGTTTTTAAGGAA	50.0	50.0	58.2	57.6	902-977 bp	0.833333	74.8
trnK-UUU-rps16_p5	AAAGCCGAGTACTCTACCGT	GGGTGGGGGTTTTTAAGGAA	50.0	50.0	58.2	57.6	903-978 bp	0.833333	74.7