Hotspot

psbC

LSC · 36391-37812 · 1422 bp

Back to Disanthus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GTGCAGCAGAAGATCCTGAA	TACTTCAAGCAGTGGATCCC	50.0	50.0	57.9	56.9	1543 bp	1.0	70.5
psbC_p2	GTGCAGCAGAAGATCCTGAA	CCTACTTCAAGCAGTGGATCC	50.0	52.4	57.9	58.1	1545 bp	1.0	70.5
psbC_p3	GTGCAGCAGAAGATCCTGAA	CTACTTCAAGCAGTGGATCCC	50.0	52.4	57.9	58.1	1544 bp	1.0	70.5
psbC_p4	AAATCCGTGCAGCAGAAGAT	TACTTCAAGCAGTGGATCCC	45.0	50.0	57.9	56.9	1549 bp	1.0	70.4
psbC_p5	AAATCCGTGCAGCAGAAGAT	CTACTTCAAGCAGTGGATCCC	45.0	52.4	57.9	58.1	1550 bp	1.0	70.3