Hotspot

psbA

LSC · 403-1464 · 1062 bp

Back to Disanthus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCAAGAGAGTTGGTATTGCTCC	TGCTTGGGAGTCCCTGATAA	45.5	50.0	57.8	58.0	1164 bp	1.0	62.1
psbA_p2	CAAGAGAGTTGGTATTGCTCCT	TGCTTGGGAGTCCCTGATAA	45.5	50.0	57.8	58.0	1163 bp	1.0	62.0
psbA_p3	TCAAGAGAGTTGGTATTGCTCC	ATGCTTGGGAGTCCCTGATA	45.5	50.0	57.8	57.8	1165 bp	1.0	60.8
psbA_p4	TCAAGAGAGTTGGTATTGCTCC	TATGCTTGGGAGTCCCTGAT	45.5	50.0	57.8	57.8	1166 bp	1.0	60.8
psbA_p5	CAAGAGAGTTGGTATTGCTCCT	ATGCTTGGGAGTCCCTGATA	45.5	50.0	57.8	57.8	1164 bp	1.0	60.7