Hotspot

matK

LSC · 2266-3537 · 1272 bp

Back to Disanthus

Pi 0.000786

MarkerSeek score 59.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1272
variable_sites: 1
pi: 0.000786
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCGAGCACAAGAAAGTCGAA	CCTTGTTTTGACTGTATCGCA	45.0	42.9	57.5	56.8	1380-1381 bp	1.0	58.7
matK_p2	TTCGAGCACAAGAAAGTCGA	CCTTGTTTTGACTGTATCGCA	45.0	42.9	57.5	56.8	1381-1382 bp	1.0	58.7
matK_p3	TCGAGCACAAGAAAGTCGAA	ACCTTGTTTTGACTGTATCGC	45.0	42.9	57.5	56.8	1381-1382 bp	1.0	58.7
matK_p4	TTCGAGCACAAGAAAGTCGA	ACCTTGTTTTGACTGTATCGC	45.0	42.9	57.5	56.8	1382-1383 bp	1.0	58.7
matK_p5	TCGAGCACAAGAAAGTCGAA	CCTTGTTTTGACTGTATCGCAC	45.0	45.5	57.5	58.2	1380-1381 bp	1.0	58.5