Hotspot

clpP

LSC · 72836-74794 · 1959 bp

Back to Disanthus

Pi 0

MarkerSeek score 58.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1959
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GATTTGCGTGAAATCCCTGC	TCCATTGTTACGTTTCCAAATCA	50.0	34.8	58.1	57.0	2040-2046 bp	1.0	47.3
clpP_p2	TTTGCGTGAAATCCCTGCTA	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.8	57.0	2038-2044 bp	1.0	46.5
clpP_p3	GATTTGCGTGAAATCCCTGC	ACGTTTCCAAATCAAAGTGA	50.0	35.0	58.1	53.6	2031-2037 bp	1.0	44.7
clpP_p4	TGCGTGAAATCCCTGCTATT	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.6	57.0	2036-2042 bp	1.0	44.7
clpP_p5	TTGCGTGAAATCCCTGCTAT	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.6	57.0	2037-2043 bp	1.0	44.7